Biodetoxification of fungal mycotoxins zearalenone by engineered probiotic bacterium Lactobacillus reuteri with surface-displayed lactonohydrolase.
Identifieur interne : 000609 ( Main/Exploration ); précédent : 000608; suivant : 000610Biodetoxification of fungal mycotoxins zearalenone by engineered probiotic bacterium Lactobacillus reuteri with surface-displayed lactonohydrolase.
Auteurs : Feixia Liu [République populaire de Chine] ; Wanna Malaphan [Thaïlande] ; Fuguo Xing [République populaire de Chine] ; Bo Yu [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2019.
Descripteurs français
- KwdFr :
- Ascomycota (enzymologie), Ascomycota (génétique), Cellules Caco-2 (MeSH), Génie génétique (MeSH), Humains (MeSH), Hydrolases (métabolisme), Inactivation métabolique (MeSH), Lactobacillus reuteri (enzymologie), Lactobacillus reuteri (génétique), Lactobacillus reuteri (métabolisme), Lignée cellulaire tumorale (MeSH), Phosphofructokinase-2 (métabolisme), Probiotiques (MeSH), Zéaralénone (métabolisme).
- MESH :
- enzymologie : Ascomycota, Lactobacillus reuteri.
- génétique : Ascomycota, Lactobacillus reuteri.
- métabolisme : Hydrolases, Lactobacillus reuteri, Phosphofructokinase-2, Zéaralénone.
- Cellules Caco-2, Génie génétique, Humains, Inactivation métabolique, Lignée cellulaire tumorale, Probiotiques.
English descriptors
- KwdEn :
- Ascomycota (enzymology), Ascomycota (genetics), Caco-2 Cells (MeSH), Cell Line, Tumor (MeSH), Genetic Engineering (MeSH), Humans (MeSH), Hydrolases (metabolism), Inactivation, Metabolic (MeSH), Lactobacillus reuteri (enzymology), Lactobacillus reuteri (genetics), Lactobacillus reuteri (metabolism), Phosphofructokinase-2 (metabolism), Probiotics (MeSH), Zearalenone (metabolism).
- MESH :
- chemical , metabolism : Hydrolases, Phosphofructokinase-2, Zearalenone.
- enzymology : Ascomycota, Lactobacillus reuteri.
- genetics : Ascomycota, Lactobacillus reuteri.
- metabolism : Lactobacillus reuteri.
- Caco-2 Cells, Cell Line, Tumor, Genetic Engineering, Humans, Inactivation, Metabolic, Probiotics.
Abstract
Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydrolase from Rhinocladiella mackenziei CBS 650.93 (RmZHD) in L. reuteri via secretion and surface-display patterns, respectively. Endogenous signal peptides from L. reuteri were first screened to achieve high expression for efficient ZEN hydrolysis. For secretion expression, signal peptide from collagen-binding protein showed the best performance, while the one from fructose-2,6-bisphosphatase worked best for surface-display expression. Both of the engineered strains could completely hydrolyze 5.0 mg/L ZEN in 8 h without detrimental effects on bacterial growth. The acid and bile tolerance assay and anchoring experiment on Caco-2 cells indicated both of the abovementioned engineered strains could survive during digestion and colonize on intestinal tract, in which the surface-displayed strain had a better performance on ZEN hydrolysis. Biodetoxification of model ZEN-contaminated maize kernels showed the surface-displayed L. reuteri strain could completely hydrolyze 2.5 mg/kg ZEN within 4 h under low water condition. The strain could also efficiently detoxify natural ZEN-contaminated corn flour in the in vitro digestion model system. The colonized property, survival capacity, and the efficient hydrolysis performance as well as probiotic functionality make L. reuteri strain an ideal host for detoxifying residual ZEN in vivo, which shows a great potential for application in feed industry.
DOI: 10.1007/s00253-019-10153-1
PubMed: 31628520
Affiliations:
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uniqKey="Malaphan W" first="Wanna" last="Malaphan">Wanna Malaphan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900</wicri:regionArea><wicri:noRegion>10900</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Fuguo" sort="Xing, Fuguo" uniqKey="Xing F" first="Fuguo" last="Xing">Fuguo Xing</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Food Science and Technology, Chinese 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Liu</name><affiliation wicri:level="1"><nlm:affiliation>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101</wicri:regionArea><wicri:noRegion>100101</wicri:noRegion></affiliation></author><author><name sortKey="Malaphan, Wanna" sort="Malaphan, Wanna" uniqKey="Malaphan W" first="Wanna" last="Malaphan">Wanna Malaphan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900</wicri:regionArea><wicri:noRegion>10900</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Fuguo" sort="Xing, Fuguo" uniqKey="Xing F" first="Fuguo" last="Xing">Fuguo Xing</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yu, Bo" sort="Yu, Bo" uniqKey="Yu B" first="Bo" last="Yu">Bo Yu</name><affiliation wicri:level="1"><nlm:affiliation>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. yub@im.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101</wicri:regionArea><wicri:noRegion>100101</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (enzymology)</term><term>Ascomycota (genetics)</term><term>Caco-2 Cells (MeSH)</term><term>Cell Line, Tumor (MeSH)</term><term>Genetic Engineering (MeSH)</term><term>Humans (MeSH)</term><term>Hydrolases (metabolism)</term><term>Inactivation, Metabolic (MeSH)</term><term>Lactobacillus reuteri (enzymology)</term><term>Lactobacillus reuteri (genetics)</term><term>Lactobacillus reuteri (metabolism)</term><term>Phosphofructokinase-2 (metabolism)</term><term>Probiotics (MeSH)</term><term>Zearalenone (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (enzymologie)</term><term>Ascomycota (génétique)</term><term>Cellules Caco-2 (MeSH)</term><term>Génie génétique (MeSH)</term><term>Humains (MeSH)</term><term>Hydrolases (métabolisme)</term><term>Inactivation métabolique (MeSH)</term><term>Lactobacillus reuteri (enzymologie)</term><term>Lactobacillus reuteri (génétique)</term><term>Lactobacillus reuteri (métabolisme)</term><term>Lignée cellulaire tumorale (MeSH)</term><term>Phosphofructokinase-2 (métabolisme)</term><term>Probiotiques (MeSH)</term><term>Zéaralénone (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrolases</term><term>Phosphofructokinase-2</term><term>Zearalenone</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Ascomycota</term><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Ascomycota</term><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hydrolases</term><term>Lactobacillus reuteri</term><term>Phosphofructokinase-2</term><term>Zéaralénone</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Caco-2 Cells</term><term>Cell Line, Tumor</term><term>Genetic Engineering</term><term>Humans</term><term>Inactivation, Metabolic</term><term>Probiotics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules Caco-2</term><term>Génie génétique</term><term>Humains</term><term>Inactivation métabolique</term><term>Lignée cellulaire tumorale</term><term>Probiotiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydrolase from Rhinocladiella mackenziei CBS 650.93 (RmZHD) in L. reuteri via secretion and surface-display patterns, respectively. Endogenous signal peptides from L. reuteri were first screened to achieve high expression for efficient ZEN hydrolysis. For secretion expression, signal peptide from collagen-binding protein showed the best performance, while the one from fructose-2,6-bisphosphatase worked best for surface-display expression. Both of the engineered strains could completely hydrolyze 5.0 mg/L ZEN in 8 h without detrimental effects on bacterial growth. The acid and bile tolerance assay and anchoring experiment on Caco-2 cells indicated both of the abovementioned engineered strains could survive during digestion and colonize on intestinal tract, in which the surface-displayed strain had a better performance on ZEN hydrolysis. Biodetoxification of model ZEN-contaminated maize kernels showed the surface-displayed L. reuteri strain could completely hydrolyze 2.5 mg/kg ZEN within 4 h under low water condition. The strain could also efficiently detoxify natural ZEN-contaminated corn flour in the in vitro digestion model system. The colonized property, survival capacity, and the efficient hydrolysis performance as well as probiotic functionality make L. reuteri strain an ideal host for detoxifying residual ZEN in vivo, which shows a great potential for application in feed industry.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31628520</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>103</Volume><Issue>21-22</Issue><PubDate><Year>2019</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Biodetoxification of fungal mycotoxins zearalenone by engineered probiotic bacterium Lactobacillus reuteri with surface-displayed lactonohydrolase.</ArticleTitle><Pagination><MedlinePgn>8813-8824</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-019-10153-1</ELocationID><Abstract><AbstractText>Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydrolase from Rhinocladiella mackenziei CBS 650.93 (RmZHD) in L. reuteri via secretion and surface-display patterns, respectively. Endogenous signal peptides from L. reuteri were first screened to achieve high expression for efficient ZEN hydrolysis. For secretion expression, signal peptide from collagen-binding protein showed the best performance, while the one from fructose-2,6-bisphosphatase worked best for surface-display expression. Both of the engineered strains could completely hydrolyze 5.0 mg/L ZEN in 8 h without detrimental effects on bacterial growth. The acid and bile tolerance assay and anchoring experiment on Caco-2 cells indicated both of the abovementioned engineered strains could survive during digestion and colonize on intestinal tract, in which the surface-displayed strain had a better performance on ZEN hydrolysis. Biodetoxification of model ZEN-contaminated maize kernels showed the surface-displayed L. reuteri strain could completely hydrolyze 2.5 mg/kg ZEN within 4 h under low water condition. The strain could also efficiently detoxify natural ZEN-contaminated corn flour in the in vitro digestion model system. The colonized property, survival capacity, and the efficient hydrolysis performance as well as probiotic functionality make L. reuteri strain an ideal host for detoxifying residual ZEN in vivo, which shows a great potential for application in feed industry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Feixia</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Malaphan</LastName><ForeName>Wanna</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xing</LastName><ForeName>Fuguo</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Bo</ForeName><Initials>B</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-3148-0986</Identifier><AffiliationInfo><Affiliation>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. yub@im.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>5182021</GrantID><Agency>Natural Science Foundation of Beijing Municipality</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>5W827M159J</RegistryNumber><NameOfSubstance UI="D015025">Zearalenone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.105</RegistryNumber><NameOfSubstance UI="D025481">Phosphofructokinase-2</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.-</RegistryNumber><NameOfSubstance UI="D006867">Hydrolases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018938" MajorTopicYN="N">Caco-2 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006867" MajorTopicYN="N">Hydrolases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008658" MajorTopicYN="N">Inactivation, Metabolic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052200" MajorTopicYN="N">Lactobacillus reuteri</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025481" MajorTopicYN="N">Phosphofructokinase-2</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019936" MajorTopicYN="N">Probiotics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015025" MajorTopicYN="N">Zearalenone</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lactobacillus reuteri</Keyword><Keyword MajorTopicYN="N">Lactonohydrolase</Keyword><Keyword MajorTopicYN="N">Surface display</Keyword><Keyword MajorTopicYN="N">Zearalenone</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31628520</ArticleId><ArticleId IdType="doi">10.1007/s00253-019-10153-1</ArticleId><ArticleId IdType="pii">10.1007/s00253-019-10153-1</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>Thaïlande</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Feixia" sort="Liu, Feixia" uniqKey="Liu F" first="Feixia" last="Liu">Feixia Liu</name></noRegion><name sortKey="Xing, Fuguo" sort="Xing, Fuguo" uniqKey="Xing F" first="Fuguo" last="Xing">Fuguo Xing</name><name sortKey="Yu, Bo" sort="Yu, Bo" uniqKey="Yu B" first="Bo" last="Yu">Bo Yu</name></country><country name="Thaïlande"><noRegion><name sortKey="Malaphan, Wanna" sort="Malaphan, Wanna" uniqKey="Malaphan W" first="Wanna" last="Malaphan">Wanna Malaphan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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